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The primers used in this study are listed in Table 1.To construct the trxH Ep knockout strain, TX01∆trxH, in-frame deletion of a 261 bp segment (residues 24 to 110) of trxH Ep was performed by overlap extension PCR as follows: the first overlap PCR was performed with the primer pair TrxHF1/R1, the second overlap PCR was performed ...Texas Roadhouse is a legendary steak restaurant serving American cuisine from the best steaks and ribs to made-from-scratch sides & fresh-baked rolls.Careers. We're HIRING! Seeking all positions. Apply online today! Join our Team - Live! Hospitality & Entertainment. Seeking fun and motivated hospitality professionals! Texas Live! is the center for Arlington's sports, dining and entertainment. 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